International Journal of TROPICAL DISEASE & Health http://www.journalijtdh.com/index.php/IJTDH <p style="text-align: justify;"><strong>International Journal of TROPICAL DISEASE &amp; Health (IJTDH) (ISSN: 2278 – 1005)</strong> aims to publish&nbsp;high quality papers (<a href="/index.php/IJTDH/general-guideline-for-authors">Click here for Types of paper</a>)&nbsp;in the areas of tropical medicine and public health research, reports on the efficacy of new drugs and methods of treatment, prevention and control methodologies, new testing methods and equipment. This is a quality controlled, peer-reviewed, open access INTERNATIONAL journal. IJTDH will not only publish traditional full research reports, including short communications, but also this journal will publish reports/articles on all stages of the research process like study protocols, pilot studies and pre-protocols. IJTDH is novelty attracting, open minded, peer-reviewed medical periodical, designed to serve as a perfectly new platform for both mainstream and new ground shaking works as long as they are technically correct and scientifically motivated. This journal has no connection with any society or association, related to Tropical medicine, disease or Public health and allied fields. This is an independent journal run by SDI.</p> SCIENCEDOMAIN international en-US International Journal of TROPICAL DISEASE & Health 2278-1005 First Evidence of West Nile Virus in Hodeidah, Yemen: Clinical and Epidemiological Characteristics http://www.journalijtdh.com/index.php/IJTDH/article/view/30190 <p><strong>Introduction</strong><strong>:</strong> West Nile Virus (WNV) infection is an important arthropod-borne zoonosis viral disease. This virus is neglected in Yemen especially in Hodeidah.</p> <p><strong>Aim of the Study</strong><strong>:</strong> The purpose of this study was to detect WNV infection, determine the epidemiological and clinical characteristics within febrile patients in Hodeidah city and to determine some risk factors associated with WNV infection.</p> <p><strong>Materials and Methods: </strong>136 febrile patients in a hospital base study were diagnosed in Center of Tropical Medicine and Infectious Diseases (CTMID), Authority of General Al-Thawara Hospital, Hodeidah, Yemen from January of 2017 to December of 2017. WNV infection was detected by enzyme linkage immune sorbent assay (ELISA) on serum samples.</p> <p><strong>Results and Discussion</strong><strong>:</strong> The results showed that 5 cases (3.67%) were WNV – positive namely IgM that was detected in winter and spring seasons, the most prevalent antibodies of WNV were IgG namely 75 cases (55.14%). Most common symptoms were fever, headache, fatigue, weakness, arthralgia, myalgia and photophobia. The treatment based on the intravenous therapy (IV) with anti-pyritic, plasma in some cases and all cases were recovered while mortality rate was 00%.</p> <p><strong>Conclusion</strong><strong>:</strong> WNV was detected in Hodeidah which placed in Tehama "western Yemen", as first time by our preliminary study that confirmed the evidence of WNV IgM and IG antibodies presence on 2017, in order to increase safety of diagnosis of febrile diseases, it is essential to continue surveillance of this emerging infection, suggesting that this emergence has been transported by migratory birds from wintering areas to Tehama region.</p> Qais Yusuf Isra'a Al-Masrafi Anas Al-Mahbashi Asma'a Al-Areeqi Mohammed Amood Al-Kamarany Ali S. Khan ##submission.copyrightStatement## 2019-10-02 2019-10-02 1 9 10.9734/ijtdh/2019/v38i430190 Evaluation of Loop Mediated Isothermal Amplification (LAMP) for Diagnosis of Plasmodium falciparum in Wad Medani City, Gezira State, Sudan http://www.journalijtdh.com/index.php/IJTDH/article/view/30191 <p><em>Plasmodium falciparum</em> considered as the most serious form of species causes malaria compared with other species. Diagnosis of <em>falciparum</em> malaria in Sudan remain a major problem, the laboratory diagnosis depends solely on microscopy and RDTs. Loop mediated isothermal amplification (LAMP) assay is a molecular technique done in isothermal temperature using simple, inexpensive instruments for detection of <em>falciparum</em> malaria. The aim of the study is to evaluate the diagnostic performance of loop mediated isothermal amplification (LAMP) assay for detection of<em> P. falciparum</em> and compare with microscopic detection<em>. </em>A cross sectional hospital based study conducted on 220 blood samples collected from participants suspected to have falciparum malaria attending Wad Medani Teaching Hospitals and 26 healthy participants during the period November 2018 to January 2019. Thick blood films were done and used for <em>P. falciparum </em>detection. The extracted DNA by TE buffer was amplified by LAMP assay targeting 18S rRNA gene. Data were analyzed using Medical calculator (MedCalc) programs (V. 16). The results showed that the sensitivity<strong>, </strong>specificity, positive predictive value, negative predictive values were 99.1%, 84.6%, 53.2%, 99.8% respectively. Validation of LAMP diagnostic performance revealed that area under the curve is 0.919, while Weighted Kappa is 0.866. The study concluded that the LAMP assay had the identical diagnostic performance compared with microscopy in diagnosis of <em>Plasmodium falciparum </em>malaria. This gives a relative effortlessness application of LAMP assay in Sudan after availing the required logistics.</p> M. Y. Mohamed A. D. Abakar B. A. Talha Salah Eldin G. Elzaki Y. A. Mohammed Usama Abdalla Elsharief K. A. Mohamed Ahmed B. Y. M. Nour ##submission.copyrightStatement## 2019-10-14 2019-10-14 1 7 10.9734/ijtdh/2019/v38i430191