Effectiveness of Three Fruit Seed Extracts as Larvicide against Three Major Mosquito Vectors Aedes aegypti Linnaeus, Culex quinquefasciatus Say and Anopheles gambiae Giles (Diptera: Culicidae)
Lame Younoussa *
Department of Animal Biology and Physiology, Faculty of Science, University of Yaounde 1, P.O. Box 812, Yaounde, Cameroon.
Kary Mallam Oumarou
School of Veterinary Medicine and Sciences, University of Ngaoundere P.O. Box 454, Ngaoundere, Cameroon.
Theodora Kopa Kowa
Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaoundé, Cameroon.
Serge Eteme Enama
Department of Animal Biology and Physiology, Faculty of Science, University of Yaounde 1, P.O. Box 812, Yaounde, Cameroon.
Gabriel Agbor Agbor
Centre for Research on Medicinal Plants and Traditional Medicine, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaoundé, Cameroon.
Elias Nchiwan Nukenine
Department of Biological Sciences, Faculty of Science, University of Ngaoundere, P.O. Box 454, Ngaoundere, Cameroon.
*Author to whom correspondence should be addressed.
Abstract
The CH2Cl2-MeOH (30:70 v/v) extracts of the seeds of Mangifera indica (Mango), Persea americana (Avocado) and Dacryodes edulis (African plum) were evaluated for potential mosquito larvicidal activity against 3rd and 4th instar larvae of Aedes aegypti, Culex quinquefasciatus and Anopheles gambiae. Extracts were diluted with 1 mL of methanol and concentrations ranging from 1000 to 125 mg/L in 4 replicates each, were prepared in the volume of 100 mL in the plastic cups (250 mL). A volume of 1 mL of methanol added to 99 mL of tap water was prepared as negative control and Bi-one (1000 mg/L) constituted a positive control. In each test solution, 25 larvae of each mosquito species were separately transferred and larval mortality was recorded after 24 h post-treatment. As results, the three plant seed extracts applied at 1000 mg/L caused for at least 79% mortality of each mosquito species larvae assessed. The seed extract of P. americana (LC50 of 98.31, 129.24 and 136.26 mg/L, respectively against An. gambiae, Ae. aegypti and Cx. quiquefasciatus larvae) was the most potent followed by D. edulis (LC50 of 176.87 mg/L for An. gambiae, 198.68 mg/L for Ae. aegypti and 201.70 mg/L for Cx. quinquefasciatus) and M. indica (LC50 of 258.98 mg/L for An. gambiae, 297.35 mg/L for Ae. aegypti and 435.45 mg/L for Cx. quinquefasciatus). Globally, all the seed extracts were more toxic against An. gambiae larvae compared to other mosquito species and need further exploration for the development of a new botanical larvicide to reduce mosquito densities.
Keywords: Larvicidal, CH2Cl2-MeOH seed extracts, mosquito species, Anopheles gambiae.