Antibacterial and Antifungal Activity of Methanolic Leaf Extract of Allium sativum on Selected Pathogenic Strains
Osheke Shekins Okere
Department of Biochemistry, Bingham University, P.M.B 005, Karu, Nigeria.
Janet Olayemi Sangodele *
Department of Biochemistry, Bingham University, P.M.B 005, Karu, Nigeria.
Moses Dele Adams
Department of Biochemistry, Bingham University, P.M.B 005, Karu, Nigeria.
Eunice Ogunwole
Department of Physiology, Bingham University, P.M.B. 005, Karu, Nigeria.
Mercy Omoye Shafe
Department of Physiology, Bingham University, P.M.B. 005, Karu, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Allium sativum plants contain chemicals which can inhibit the growth of microorganisms and thus make them suitable to be used in different medicines. The antibacterial and antifungal activities of Allium sativum methanolic extracts were investigated using standard analytical techniques and modern micro plate-based antibacterial assays techniques. The antibacterial of methanolic extract of Allium sativum were used on different bacteria strains which include Escherichia coli, Pseudomonas aeruginosa, Protea spp., Salmonella typhi and Staphylococcus aureus. The antifungal observation of Allium sativum was found to be active against Aspergilus flavus, Aspergilus niger, Aspergilus fumigatus, Penicillium spp compared to Funbact-A (control). The susceptibility test showed that Allium sativum is more active against Aspergilus fumigatus. The Allium sativum had a higher zone of inhibition compared to Ciproflaxin (control) on the different bacteria strains. Aspergillus fumigatus and Aspergillus niger differs significantly in their degree of inhibition of methanolic extract of Allium sativum. The findings support the use of Allium sativum extract in treating bacterial infections and Aspergillosis.
Keywords: Allium sativum, antibacterial, antifungal, ciproflaxin, zone of inhibition