Genetic and Phylogenetic Characterization of Dengue Virus among Clinically Suspected Patients in Eastern India
Namrata Kumari
Molecular Biology, Virology, MDR-TB and Bacteriology, Blood Bank Department of Microbiology IGIMS Patna, India.
Rishikesh Kumar *
Molecular Biology, Virology, MDR-TB and Bacteriology, Blood Bank Department of Microbiology IGIMS Patna, India.
Nidhi Prasad
Molecular Biology, Virology, MDR-TB and Bacteriology, Blood Bank Department of Microbiology IGIMS Patna, India.
Kalyani Mishra
Viral Diagnostic Research Laboratory and Department of Bio-informatics RMRIMS, Patna, India.
Khushboo Kumari
Viral Diagnostic Research Laboratory and Department of Bio-informatics RMRIMS, Patna, India.
Ganesh Chandra Sahoo
Viral Diagnostic Research Laboratory and Department of Bio-informatics RMRIMS, Patna, India.
Sudhir Kumar
Department of General Medicine, IGIMS Patna, India.
Abhay Kumar
Molecular Biology, Virology, MDR-TB and Bacteriology, Blood Bank Department of Microbiology IGIMS Patna, India.
Govind Prasad
Department of General Medicine, IGIMS Patna, India.
Naresh Kumar
Department of General Medicine, IGIMS Patna, India.
Rakesh Kumar
Molecular Biology, Virology, MDR-TB and Bacteriology, Blood Bank Department of Microbiology IGIMS Patna, India.
Tejaswini Prasad Babar
Department of Dravyaguna, Bharti Vidyapeeth Pune, India.
Shailesh Kumar
Molecular Biology, Virology, MDR-TB and Bacteriology, Blood Bank Department of Microbiology IGIMS Patna, India.
Krishna Pandey
Viral Diagnostic Research Laboratory and Department of Bio-informatics RMRIMS, Patna, India.
*Author to whom correspondence should be addressed.
Abstract
AIM: Dengue is an acute systemic viral disease well known globally in both endemic and epidemic transmission cycles. Main aim is to study serotypes/genotypes and lineages of dengue virus (DENV) are associated with more severe outbreaks. Many reports from India have shown an association between change in DENV genotype/lineage and magnitude of the outbreak and disease severity.
Study Design. It is a hospital based cross sectional study.
Place and Duration of Study: Molecular Biology laboratory in department of Microbiology, Indira Gandhi Institute of Medical Science and duration study from January2021 to January 2023.
Material and Methods: This cross- sectional study was aimed to investigate the circulating DENV serotypes and genotypes in Bihar during year 2021-2023. For genetic characterization of prevalent serotypes, real-time reverse transcription polymerase chain reaction assay was used. Representative samples were sequenced for the envelope (E) gene.
Results: All four prevalent serotypes, DENV-1, DENV-2, DENV-3 and DENV-4 were found to be circulating in Bihar with dominance of DENV-2. Mixed infection cases of DENV-2 with DENV-3 and DENV-1 with DENV-2 were also seen. Phylogenetic analysis based on C-prM gene revealed DENV-1 sequences to be 92% similar with Vietnam genotype I (2003) strain, DENV-2 isolates clustered with genotype IV of North India strains, Haryana (1996), Delhi (1996) and Jammu (1993) with 95%, 94% and 94% sequence similarity respectively. DENV-3 isolate was more closely related to genotype III of Indian origin (2003), Gwalior and Delhi with 98% and 99% sequence similarity respectively.
Conclusion: With this background data, molecular monitoring of viruses circulating in the locality provides baseline data on the circulating serotypes/ genotypes of dengue virus (DENV). So this monitoring and early detection of changes in the circulation pattern may help in the prediction of DENV outbreaks and can augment disease control efforts.
Keywords: Dengue virus, genetic characterization, dengue epidemiology, genomic sequences